Spring 2011
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Syllabus
Lab Exercises
01 Tools of the Trade
02 Growth of E. coli Bacteria
03 Isolation and Titration of Bacteriophage Lambda
04 Gel Electrophoresis
05 Transformation of E. coli with Plasmid DNA
06 Purification and restriction analysis of plasmid DNA
07 Isolation of green fluorescent protein (GFP) by Chromatography and PAGE
08 Mutagenesis of pGlo plasmid by Restriction digest
09 PCR of Alu DNA from Human Cells
10 Enzyme-Linked Immunosorbant Assay
11 C. elegans Genetics
12 Yeast Genetics
 
 
 
 
 
 
 
 
 
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