Composition of Blood

Typically, males have about 5.5 liters of blood and females 5 liters of blood.

Blood is made of three specialized elements called erythrocytes (red blood cells), leukocytes (white blood cells) and platelets that are suspended in a complex liquid called plasma. The constant flow of blood keeps the cells evenly distributed in the plasma.

Most of the cells in the blood (about 99%) are erythrocytes (the red blood cells or RBCs that carry oxygen). The leukocytes (white blood cells or WBCs) and the platelets make up less than about 1% of the cells found in blood.

Plasma consists of about 9O% water with the other 10% being plasma proteins, organic and inorganic compounds, and electrolytes (ions).

The plasma proteins of blood serve a variety of functions including:
1. help maintain the osmotic pressure of the blood.
2. partially assist in regulating blood pH.
3. binding to and transporting substances.
4. some plasma proteins are immunoglobins (antibodies) which are essential for the functions of the immune system.
5. A variety of plasma proteins, particularly fibrinogen, are essential for blood coagulation (the blood clotting process).

Blood Cells

Blood cells are formed in the bone marrow. All blood cells arise from the same bone marrow stem cells. Stem cells are immortal, meaning they never die (at least not until you do). Stem cells are also undifferentiated, meaning they have not yet developed into a particular cell type. Furthermore, stem cells are pluripotent, meaning they have the potential to become any type of blood cell. These immortal, undifferentiated, pluripotent stem cells give rise to erythrocytes, leukocytes and platelets. The diagram below illustrates the different types of blood cells. Leukocytes, also known as white blood cells, are a group of related cell types that are involved in immune function. Leukocytes include neutrophils, eosinophils, basophils, lymphocytes and monocytes.

Erythrocytes

Erythrocytes, also known as red blood cells (RBCs), function to transport oxygen in the blood. The shape of erythrocytes is ideal for this function. Seen from the top, erythrocytes appear to be circular, but a side view shows that they are actually biconcaved discs. This shape increases the surface area-to-volume ratio of the cell, thus increasing the efficiency of diffusion of oxygen and carbon dioxide into and out of the cell. Erythrocytes also have a flexible plasma membrane. This feature allows erythrocytes, which have a 7mm diameter, to squeeze through capillaries as small as 3 mm wide. Erythrocytes contain tremendous amounts of hemoglobin, the protein that binds oxygen. In order to make room for more hemoglobin to carry more oxygen, erythrocytes loose their nucleus and other organelles as they develop in the bone marrow. Because they lack a nucleus and other cellular machinery, erythrocytes cannot repair themselves when damaged, consequently they have a limited life span of about 120 days. The removal of old and dying erythrocytes is carryied out by the spleen. Erythrocytes, which represent the most numerous cell type in the body die at a rapid rate, 2-3 million erythrocytes die every second. Erythrocyte production must equal erythrocyte death or the cell population would decline.

Hematocrit The portion of the blood which is made up of erythrocytes is called the hematocrit. Hematocrit is usually expressed as a percentage of the total blood volume. It is the red portion of the blood that is found at the bottom of a test tube of blood that has been centrifuged. If whole blood is placed into a tube and centrifuged, the cells and the plasma will separate. The erythrocytes, which are heavy, will pack into the bottom of the tube, the plasma will be at the top of the tube, and the leukocytes and platelets will form a thin layer (buffy coat) between the erythrocytes and the plasma. The hematocrit is defined as the percentage of whole blood made up of erythrocytes. This value is determined by dividing the height of the erythrocytes by the total height of the blood in the tube and multiplying by 100.

Hematocrits vary depending on sex and environmental conditions, but there is a range of values that is considered normal. Average hematocrit values are:

Any activity or condition that consistently lowers oxygen levels in the blood will cause an increase in erythropoesis and a subsequent rise in the hematocrit.

Factors that will raise the hematocrit include:

• Exercise. During aerobic exercise blood oxygen levels are lowered due to rapid consumption of oxygen by active skeletal muscle. This stimulates an increase in erythropoesis, which increases hematocrit, which increases the oxygen carrying capacity of the blood. Thus regular aerobic exercise raises the hematocrit.
• Living at high altitude. The air is thinner at higher altitude, therefore fewer molecules of oxygen enter the lungs with each breath. Oxygen levels in the blood are lower when breathing such thin air. A person that moves from Santa Barbara, which is at sea level, to Denver, Colorado, which has an altitude of 5000', will experience a rise in hematocrit as a compensatory response to the thin air.
• Injection of recombinant erythropoetin. Some endurance athletes use erythropoetin (illegally) to increase their hematocrit as a way to increase stamina. Erythropoetin is a hormone that stimulates red blood cell formation and is sometimes used to increase the hematocrit of some cancer patients undergoing chemotherapy. Chemotherapy stops cell division and can sometimes make cancer patients anemic. By giving patients extra erythropoetin

   

Anemia

Anemia is a condition that is characterized by a reduction in the oxygen carrying capacity of the blood. This reduction is caused by inadequate levels of hemoglobin, inadequate numbers of erythrocytes (low hematocrit) or both.

Symptoms of anemia are variable, but may include:

1. Fatigue. One of the most common and debilitating symptoms of anemia is fatigue (lack of energy), particularly with exercise. Oxygen is required to metabolize fuel molecules (sugars, fats and proteins) to obtain energy. A person with a low hematocrit cannot carry enough oxygen in the blood to meet their energy demands.
2. Increased heart rate. The body increases heart rate to compensate for the low oxygen carrying capacity of the blood. If more blood is moved faster through the tissue then tissues get more oxygen per unit time.
3. Shortness of breath. An anemic person may feel short of breath and then breath faster to alleviate the feeling. This is a compensation for the poor delivery of oxygen to the tissues.
4. Low blood pressure. The viscosity of the blood drops as the hematocrit decreases. A decrease in blood viscosity directly lowers total peripheral resistance (TPR) to the flow of blood, thus lowering mean arterial blood pressure (MAP).
5. Pale Skin. Hemoglobin is bright red when oxygenated and less red when deoxygenated. Because the redness of skin is due to the redness of blood, the skin of an anemic person (who has less oxygen in the blood) will be less red (paler) than the average person.

Causes of Anemia

As mentioned earlier, anemia is characterized by either low hemoglobin, low hematocrit, or both. There are several situations that can lead to this state. The causes of anemia include:

1. Dietary deficiencies of iron, vitamin B12 or folic acid.
2. Hemorrhage
3. Hemolysis
4. Bone marrow failure
5. Kidney disease

Dietary deficiencies

• Iron is required for the production and function of hemoglobin. In the absence of adequate iron, hemoglobin production slows down. Low hemoglobin can lower the hematocrit.
• Vitamin B12 and Folic Acid are required for DNA synthesis prior to cell division. In the absence of these nutrients production of erythrocytes is reduced. The hematocrit is low and many erythrocytes are huge, fragile cells called macrocysts. B12 deficiency can be caused by a lack of intrinsic factor, this is called pernicious anemia. Intrinsic factor, which is produced in the stomach, is required for efficient absorption of B12 out of the small intestine and into the blood.

Hemorrhage refers to a significant loss of blood through bleeding. Hemorrhagic anemia is due to blood loss that is greater than the rate at which erythrocytes can be replaced. Blood loss may be due to injury, donating blood, ulcers, heavy menstruation, etc.

Hemolysis refers to the lysis (breaking) of erythrocytes. Hemolytic anemia is due to a high rate of erythrocyte lysis in the blood stream. Sickle cell anemia, which is caused by defective hemoglobin, is a genetic form of hemolytic anemia. Under conditions of low oxygen (as during exercise) the hemoglobin within erythrocytes crystallizes. This causes the RBCs to adopt a sickled shape, which makes them fragile and easily lysed.

Bone marrow failure leads to a reduction in the production of erythrocytes. This may be due to cancer or toxic drugs.

Kidney disease can lead to a reduction in the synthesis of erythropoetin, resulting in a low hematocrit.

Leukocytes

Leukocytes or white blood cells perform a variety of functions related to immunity. These cells are the primary cells responsible for the defense against foreign invaders into the body such as viruses and bacteria.

Platelets

Platelets are the cellular elements in blood that help to form blood clots. A blood clot is formed as platelets become trapped in a network of protein fibers.